Table 1 Primers used in a qRT-PCR assay for the detection of PRSV load.
Primer | Oligonucleotides (5’−3’) | Initial denaturation | PCR reaction | Amplicon length (bp) | Reference |
|---|---|---|---|---|---|
PRSV2F | GGCACACTTAGATGGAGGACC | Cycles: 1 95 °C for 30 s | Cycles: 40 95 °C for 5 s 60 °C for 30–34 s Dissociation Stage | 136 | Designed |
PRSV2R | ACCAGGAAGGTGGTAGGTTCA | ||||
TBP2F | TGTGAATACTGGTGCTGAG | 104 | Zhu et al.78 | ||
TBP2R | GGCATGAGACAAGACCTATA |
