Fig. 7 | Scientific Reports

Fig. 7

From: The new bilastine eye drop formulation protects against conjunctival dehydration and promotes corneal wound healing in a comparative in vitro study

Fig. 7

In Vitro Wound Healing Assay of Human Primary Corneal Epithelial Cells (HCEpC). After the scratch, HCEpC cells were incubated in fresh medium with or without EDF 1, EDF 2, EDF 3, EDF 6 formulations at 0.78% v/v dilution for 72 h. The wound area was imaged and quantified at 0, 12, 24, 36, 48, 60 and 72 h post-scratching. (a) Quantification of Wound Healing Activity. Wound healing rate was expressed as the percentage of the remaining wound area compared to 0 h. (b) Representative transmitted light optical microscopy images (4x objective) of wounds at 0 h, 24 h, 48 h, and 72 h (scale bar: 200 μm). Intermediate images have been omitted to avoid redundancy. EDF 1 (0.025% ketotifen fumarate); EDF 2 (0.05% azelastine hydrochloride); EDF 3 (0.6% bilastine); EDF 6 (0.776% olopatadine hydrochloride); C+: positive control; C-: negative control.

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