Fig. 3

Immunophenotyping analysis of lymphocytes in TdLNs and tumors from mice intraperitoneally administered anti-PD-1 antibodies after inoculation with LLC1 cells or MC-38 cells. Anti-PD-1 mAb or isotype control (100 μg/body, n = 5) was administered a total of 3 times to mice every 3 days from 6 days after inoculation with LLC1 cells or MC-38 cells (1 × 10⁶ cells/body). TdLNs and tumors were harvested 7 days after the final dosing, digested into cell suspensions, and analyzed by flow cytometry using fluorescence-conjugated antibodies. The average ratios of several lymphocytes, including CD4⁺T cells, CD8⁺T cells, regulatory T cells (Tregs), B cells, NK cells, and NKT cells, and non-lymphocytes in CD45⁺ cells from TdLNs (A) and tumors (B) are shown in pie charts with the respective ratios in parentheses. The average ratios of CD3⁺T cells, CD4⁺T cells, CD8⁺T cells, Tregs, B cells, NK cells, and NKT cells in CD45⁺ cells from TdLNs (C) and tumors (D) are shown in bar graphs. Bar graphs depict the average values with SD. Significant differences are denoted with asterisks (*P < 0.05, **P < 0.005).