Fig. 4

Analysis of naïve and memory CD4⁺T cells and CD8⁺T cells in TdLNs and tumors from mice intraperitoneally administered anti-PD-1 antibodies after inoculation with LLC1 cells or MC-38 cells. Anti-PD-1 mAb or isotype control (100 μg/body, n = 5) was administered a total of 3 times to mice every 3 days from 6 days after the inoculation with LLC1 cells or MC-38 cells (1 × 10⁶ cells/body). TdLNs and tumors were harvested 7 days after the final dosing, digested into cell suspensions and analyzed by flow cytometry using fluorescence-conjugated antibodies. The average ratios of CD62L⁺CD44^– (naïve), CD62L⁺CD44⁺ (central memory: T_CM), and CD62L^–CD44⁺ (effector memory: T_EM) in CD4⁺T cells or CD8⁺T cells in CD45⁺ cells from TdLNs (A, left: CD4⁺T cells, right: CD8⁺T cells) and tumors (B, left: CD4⁺T cells, right: CD8⁺T cells) are shown in bar graphs. Bar graphs depict the average values with SD. Significant differences are denoted with asterisks (*P < 0.05).