Fig. 5

Analysis for average ratios of lymphocytes expressing CD107a, granzyme B or perforin in tumors from mice intraperitoneally administered anti-PD-1 antibodies after inoculation with LLC1 cells or MC-38 cells. Anti-PD-1 mAb or isotype control (100 μg/body, n = 5) was administered a total of 3 times to mice every 3 days from 6 days after the inoculation with LLC1 cells or MC-38 cells (1 × 10⁶ cells/body). Tumors were harvested 7 days after the final dosing, digested into cell suspensions, and analyzed by flow cytometry using fluorescence-conjugated antibodies. The average ratios of CD107a⁺, granzyme B⁺ or perforin⁺ in several lymphocytes, including CD4⁺T cells (A), CD8⁺T cells (B), regulatory T cells (Tregs; C), B cells (D), NK cells (E), and NKT cells (F) in CD45⁺ cells from tumors are shown in bar graphs. Bar graphs depict the average values with SD. Significant differences in bar graphs are denoted with asterisks (*P < 0.05, **P < 0.005).