Fig. 6

Correlation analysis between tumor volume and average ratios of lymphocytes expressing CD107a, granzyme B or perforin in tumors from mice intraperitoneally administered anti-PD-1 antibodies after inoculation with LLC1 cells or MC-38 cells. Anti-PD-1 mAb or isotype control (100 μg/body, n = 5) was administered a total of 3 times to mice every 3 days from 6 days after the inoculation with LLC1 cells or MC-38 cells (1 × 10⁶ cells/body). Tumors were harvested 7 days after the final dosing, digested into cell suspensions and analyzed by flow cytometry using fluorescence-conjugated antibodies. The relationships between tumor volumes and the average ratios of CD107a⁺, granzyme B⁺ or perforin⁺ of several lymphocytes including CD4⁺T cells, CD8⁺T cells, regulatory T cells (Tregs), B cells, NK cells, and NKT cells in CD45⁺ cells in tumors from LLC1 tumor-bearing mice (A) and MC-38 tumor-bearing mice (B) are shown in scatter plots with approximation lines.