Table 3 Gaussian parameters taken into account to fit the fluorescence emission spectrum of the endogenous fluorophores excited in DUV range (275 nm) and in NIR range (810 nm and 890 nm).
NIR Fluorophores and SHG | Maximum wavelength (nm) | Spectral bandwidth (nm) |
|---|---|---|
SHG at 890 nm only | 440–450 | 0–5 |
NADH free | 460–470 | 45–50 |
NADH bound at 810 nm only | 443–445 | 40–48 |
FAD | 520–530 | 30–50 |
Lipopigments | 570–600 | 0–180 |
Porphyrins I | 615–630 | 0–10 |
Porphyrins II | 675–690 | 0–10 |
DUV Fluorophores | Maximum wavelength (nm) | Spectral bandwidth (nm) |
|---|---|---|
Tyrosine | 301–311 | 0–50 |
Tryptophan | 335–345 | 0–10 |
Collagen | 380–420 | 0–50 |
NADH | 430 | 0–60 |
