Fig. 4
From: Identification of novel COL4A5 variants and prenatal diagnosis in three large families
Minigene-assay transcript analysis. (A) Schematic representation of constructing pcMINI-COL4A5-wt/mut recombinant vectors. (B) Electrophoresis of the RT-PCR products of the minigene transcripts in 293T and Hela cells. The DNA marker (M) on the right can predict the pcMINI-COL4A5-wt (wt) and the pcMINI-COL4A5-mut (mut) amplicon sizes. The wt exhibited a full-length band and mut had a much shorter size. (C) Schematic diagram of the aberrant splicing and sanger sequencing results of the RT-PCR products.
