Table 2 Bacterial strains and plasmids used in this work.

Strains

Stenotrophomonas maltophilia

K279a (wild-type)

Clinical isolate and the genetic reference strain

Laboratory collection and ^51,66

K279a Δsmlt1522

K279a carrying a deletion in gene Smlt1522

This study

Escherichia coli

DH5a

F⁻ Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_K⁻ m_K⁺) phoA supE44 thi-1 gyrA96 relA1λ⁻

Laboratory collection and ⁶⁷

SY327

Δ(lac pro) argE(Am) recA56 rif^R nalA λ pir

⁶⁸

Origami™ B (DE3)

F^– ompT hsdSB (r_B^–, m_B^–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (Kan^r, Tet^r)

Novagen

Agrobacterium tumefaciens

KYC55

Harboring vectors pJZ384 (PT7-TraR, Spc^r), pJZ410 (RNApol T7, Gm^r), pJZ372 (PtraI-lacZ, Tc^r). AHLs-reporter strain

³⁰

Pseudomonas aeruginosa

PAO1

Clinical isolate and the genetic reference strain

Laboratory collection and ⁶⁹

Staphylococcus aureus

Newman

Clinical isolate and the genetic reference strain

Laboratory collection and ⁷⁰

Plasmids

pGPI-SceI-XCm

Mobilizable suicide vector; carries the R6Kγ origin of replication, the I-SceI recognition site and a xylE reporter gene, Cm^r, Tp^r

⁵⁶

pΔsmlt1522-US’DS’

pGPI-SceI-XCm containing the upstream and downstream flanking DNA regions of the smlt1522 gene

This study

pRK2013

RK2-derived helper plasmid carrying the tra and mob genes for mobilization of plasmids containing oriT, Kan^r

⁷¹

pDAI-SceI-SacB

Mobilizable broad host range plasmid; carries the gene for the I-SceI homing endonuclease and the sacB gene, Tet^r

^56,72

pBBR1-BAD

Broad-host-range cloning vector used for complementation, low copy, Cm^r

⁵⁸

pBBR1-BAD-smlt1522

pBBR1-BAD with the gene smlt1522 inserted between sites XbaI and HindIII, Cm^r

This study

pET22b

Bacterial expression vector with T7-lacO promoter, Amp^r

Novagen

pET22b-Smlt1522

Plasmid for Smlt1522 protein production in E. coli

This study