Fig. 3 | Scientific Reports

Fig. 3

From: MYBL2 promotes cell proliferation and inhibits cell apoptosis via PI3K/AKT and BCL2/BAX/Cleaved-caspase-3 signaling pathway in gastric cancer cells

Fig. 3

Effects of MYBL2 on apoptosis of GC cells. (A) MYBL2 silencing significantly promoted the apoptosis of HGC27 cells by flow cytometry (Annexin V/PI staining) analysis. (B) MYBL2 overexpression significantly inhibited the apoptosis of AGS cells by flow cytometry (Annexin V-PE/7-AAD staining) analysis. (C) Western blot analysis of the expression levels of apoptosis-related proteins MYBL2, BAX, BCL2, Cleaved-caspase-3 p17 and Cleaved-caspase-3 p19 after treating HGC27 cells with siRNA for 48 h, respectively. (D) Changes in the expression levels of apoptosis-related proteins MYBL2, BAX, BCL2 and Cleaved-caspase-3 p17 and Cleaved-caspase-3 p19 were analyzed by Western blotting after treating AGS cells with the overexpression vector for 48 h. *P < 0.05, **P < 0.01, ***P < 0.001 vs. NC.

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