Fig. 4 | Scientific Reports

Fig. 4

From: Oral administration of antimicrobial peptide MPX can effectively regulate LPS-mediated testicular oxidative stress and blood‒testis barrier damage

Fig. 4

Mechanism by which MPX reduces LPS-induced testicular oxidative stress. (A–D) mRNA expression of Keap1, GLCM, GCLC and iNOS in the testes at Days 20 and 40 after MPX treatment. (E) Immunohistochemical analysis of the protein expression of Nrf2 in the testes at Days 20 and 40 after MPX treatment. (F) Measurement area (%) of Nrf2 expression in the testes. (G) Immunohistochemical analysis of the protein expression of iNOS in the testes at Days 20 and 40 after MPX treatment. (H) Measurement area (%) of iNOS expression in the testes (*p < 0.05, **p < 0.01, * represented MPX treatment group vs. LPS group; #p < 0.05, ##p < 0.01, # represented Polymyxin B treatment group vs. LPS group) (Kelch-like ECH-associated protein 1,Keap1; Nuclear factor erythroid 2-related factor 2, Nrf2; GLCM glutamate cysteine ligase, modifier, GCLC glutamate cysteine ligase, catalytic, n = 10 mice/group).

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