Fig. 1
From: 70% ethanol preserves mycobacterial RNA from cultures more efficiently than GTC-TCEP
Setup of three experiments to analyse RNA preservation with GTC-TCEP and 70% ethanol. The three experiments were started independently from different LJ cultures of the same M. tuberculosis H37Ra strain, aiming for 4 mg of bacilli per aliquot. For (b) and (c) samples were extracted at baseline and after different storage conditions. Per set of technical triplicates, a negative control with sterile physiological water was taken along. LJ: Löwenstein-Jensen medium, AT: ambient temperature (ca. 20 °C), i – v: freeze-thaw scenarios, described in Materials and Methods (“Freeze-thaw challenge”) and Fig. 5.
