Table 2 Sequences of the used primers and probes

From: Accelerators of chronic hepatitis B fibrosis cirrhosis CCND1 gene expression and promoter hypomethylation

Gene

Forward primer sequence (5′-3′)

Reverse primer sequence (5′-3′)

Probe oligo sequence

Methylight

 CCND1

TTTGTCGGGTTTTGATTT

AACTCTACTACTCGCTAC

ACTTTACAACTTCAACAAAACTCCCCT

 ACTB

TGGTGATGGAGGAGGTTTAGTAAGT

AACCAATAAAACCTACTCCTCCCTTAAA

ACCACCACCCAACACACAATAACAAACACA

RT-qPCR

 CCND1

GCTGCGAAGTGGAAACCATC

CCTCCTTCTGCACACATTTGAA

 

 B-actin

ATGGGTCAGAAGGATTCCTATGTG

CTTCATGAGGTAGTCAGTCAGGTC

 
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