Table 1 Summary of purification of recombinant lipase.
From: Heterologous expression and enzymatic properties of lipase from Mucor circinelloides
Procedures | Total protein (mg) | Total activity (U) | Specific activity (U/mg protein) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
Crude extract | 162 ± 2.6 | 486 ± 11.5 | 3.0 | 100.0 | 1.0 |
Ultrafiltration | 119 ± 5.3 | 405 ± 10.2 | 3.4 | 83.3 | 1.1 |
Ethanol fraction | 82 ± 4.8 | 301 ± 9.9 | 3.7 | 61.9 | 1.2 |
Ni-Sepharose affinity chromatography | 44 ± 1.1 | 172 ± 6.4 | 3.9 | 35.4 | 1.3 |
