Fig. 1

Egg hatching inhibition assay. (A) Temporal series of the number of second-stage juveniles (J2) hatched out of three egg masses. (B) Total number of J2 hatched out at the end of the experiment and total inhibition rate of the most active extracts, from isolates 10034 (Alternaria terricola), 10070 (Alternaria alternata) and 110040 (Dothiora maculans). Egg masses were exposed for five days to the extracts diluted in DMSO:0.6% Tween 20 or DMSO:0.6% Tween 20 for controls. The number of J2 hatched out of the egg masses was recorded at day 0 (after five days of exposition to extracts) and at 7, 14, 21 and 28 days immersed in water. Data (n = 4) shows means ± standard deviations.