Table 2 Immunofluorescence stain set using 7-color Opal staining kit.

From: Localization of epidermal growth factor receptor-mutations using PNA:DNA probes in clinical specimens from patients with non-small cell lung cancer

Set No./color No

1

2

3

4

5

6

A

DAPI

PNA

CK (AE1, AE3)*

CD8

(C8/144b)

PD-1

(NAT105)

PD-L1 (28–8)

B

DAPI

PNA

CK

(AE1, AE3)

CD4

(4B12)

FoxP3 (236A/E7)

 

C

DAPI

PNA

CK

(AE1, AE3)

CD68

(3A9A7)

CD204 (KT118)

 
  1. PNA: L858R probe.
  2. *Clone name of the antibody is shown in the parenthesis.