Fig. 3 | Scientific Reports

Fig. 3

From: NOTCH pathway was involved in Kaempferol 3-O-gentiobioside attenuated airway inflammation and mucus hypersecretion

Fig. 3

K3G Inhibits Mucus Production in IL-13-Activated 16HBE Cells. (A) The mRNA levels and (B) protein concentrationsof MUC5AC were determined via qRT-PCR and ELISA, respectively. (C) Quantitative analysis of MUC5AC immunofluorescence. #P < 0.05, ##P < 0.01, and ###P < 0.001 compared to Ctrl; *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the IL-13-treated group. (D) Fluorescence staining was utilized to observe the distribution of MUC5AC in 16HBE cells treated with IL-13 alone, or in combination with K3G (5, 25, or 50 µM). MUC5AC is indicated by red fluorescence. Scale bar: 50 μm.

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