Fig. 4 | Scientific Reports

Fig. 4

From: NOTCH pathway was involved in Kaempferol 3-O-gentiobioside attenuated airway inflammation and mucus hypersecretion

Fig. 4

K3G Inhibits IL-13-Triggered Activation of the NOTCH Pathway in 16HBE Cells. (A–E) The protein levels of NOTCH1-3 and DLL4 were analyzed and quantified via Western blot. (F–I) The mRNA expressions of NOTCH1-3 and DLL4 were evaluated by qRT-PCR. #P < 0.05, ##P < 0.01, and ###P <0.001 compared to the Ctrl group; *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the IL-13-treated group. The K3G 50 group is the group that received a high dose of K3G (50 µM) and was not treated with IL-13. (J) Molecular docking analysis explored the binding properties of K3G to NOTCH1. (K) Biacore X100 was employed to identify the kinetic and affinity parameters of the K3G-NOTCH1 interaction.

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