Fig. 5

Effects of K3G on the TLR4/NF-κB/NLRP3 Signaling Pathway. (A–E) The expression levels of TLR4, NLRP3, p-IκBα and p-P65were analyzed by immunoblotting. (F) Quantitative analysis of NF-κB/p-65 immunofluorescence. (G) Immunofluorescence staining images of NF-κB/P65 in 16HBE cells under different experimental conditions. NF-κB/P65 is shown in red, and Hoechst 33,342 in blue. Images represent 5 experiments. ##P < 0.01, ###P < 0.001 compared to the Ctrl group; *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the IL-13-treated group. Scale bar: 100 μm.