Fig. 7 | Scientific Reports

Fig. 7

From: NOTCH pathway was involved in Kaempferol 3-O-gentiobioside attenuated airway inflammation and mucus hypersecretion

Fig. 7

K3G Reduces Mucus Overproduction and NOTCH Pathway Activation in OVA-Induced Mice. (A) Representative tissue sections of lungs stained with PAS from each group (n = 6). Scale bar: 10 μm. (B) Immunofluorescence staining displays the distribution of MUC5AC in mouse lung sections, indicated by green fluorescence. Scale bar: 100 μm. (C) PAS staining was quantitatively assessed. (D) MUC5AC mRNA levels in lung sections were determined by qRT-PCR. (E) MUC5AC protein concentrations were measured by ELISA. (F-J) Protein levels of NOTCH1-3 and DLL4 were analyzed and quantified through Western blotting. #P < 0.05, ##P < 0.01, and ###P < 0.001 compared to Ctrl; *P < 0.05, **P < 0.01, and  ***P < 0.001 compared to the OVA-treated group.

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