Fig. 5

Tellurite impact in bacterial stress response and glucose consumption and importance of nitrogen source for growth of Bacillus altitudinis strain 3W19 in the presence of Te(IV). (a) ROS production assay showing two incubation periods with H₂DCFDA. Relative Fluorescence Units (RFU) or the ratio between the fluorescence of the sample (test) and the fluorescence of the control experiment. One-way ANOVA was used to compare each condition variation through time and statistical analysis is indicated as p-value ** p ≤ 0.01, (b) Glucose quantification over time, measured by DNS reagent oxidation, of strain 3W19 grown in the conditions: Control [no Te(IV)] and 0.5 mM Te(IV), 2wayANOVA multiple comparison analysis performed between each time and time 0 h in control and 0.5 mM Te(IV) and statistical analysis represented as p-value: a - p ≤ 0.05, b - p < 0.0001; ns - p > 0.05. (c) Bacterial growth in the control and 0.5 mM Te(IV) with variations of concentration of N sources in the growth media. Horizontal coloured bars indicate median value, 2wayANOVA multiple comparison analysis performed between all conditions and statistical analysis is indicated as p-values: * p ≤ 0.05, **** p < 0.0001. Three biological replicates (n = 3) were performed in each assay and in panels (a) and (b) error bars indicate standard deviation.