Fig. 5

Observation of NETs formation in vitro. (a): Trypan blue staining of isolated neutrophils; (b) and (c): Expressions of CD11b and ly6G; (d): MPO-DNA concentration after adding different dosage of Indomethacin. bar: mean ± SE, n = 3; vs control group, ^**P < 0.01; (e): NETs marker expressions in vitro (× 60, scale bar = 20 µm), red indicates H3Cit, green indicates MPO, and blue indicates DAPI-stained nucleus; (f): Fluorescence intensity of H3Cit in vitro, bar: mean ± SE, n = 3; (g): Fluorescence intensity of MPO, bar: mean ± SE, n = 3; (h): Fluorescence intensity of co-expression of MPO and H3Cit, bar: mean ± SE, n = 3. vs control group, ^**P < 0.01, ^*P < 0.05, vs model group, ^##P < 0.01.