Fig. 6

Observation of programmed cell death of GECs. (a): Observation of ultrastructure of GECs (×30,000, scale bar = 500 nm), N: nucleus, Mi: mitochondrion, RER: rough endoplasmic reticulum; : normal mitochondrion,
: expanded rough endoplasmic reticulum,
: mitochondrial swelling,
: perforation of cell membranes; (b): Relative expressions of Apaf-1 and Caspase-1 mRNA. bar: mean ± SE, n = 4; (c): Relative expressions of Gasdermin D and Cyto C proteins. bar: mean ± SE, n = 4; (d): Relative expressions of Gasdermin D and Cyto C mRNA. bar: mean ± SE, n = 4; vs control group, **P < 0.01, *P < 0.05.