Table 1 Information on 11 candidate reference genes, primers, and amplification characteristics note: E, amplification efficiency; R², regression coefficient.

From: Screening and validation of optimal real-time PCR reference genes for Abelmoschus Manihot

Gene symbol	Unigene annotation	Primer sequence (5′-3′)	Product (bp)	E (%)	R²
ACT2	Actin 2	ACACTGTGCCAATCTATGAAG	145	101.6	0.999
ACT2	Actin 2	ACAATTTCCCGCTCAGCAGTG	145	101.6	0.999
PP2A1	Protein phosphatase 2 A	GACATCATGTCCATGTTTGATG	149	98.8	0.997
PP2A1	Protein phosphatase 2 A	TGTGAGAAATTAACAATGACAG	149	98.8	0.997
PP2A2	Catalytic subunit of protein phosphatase 2 A	ATCGGAGGCGGAGGTGAAGAC	141	92.3	0.996
PP2A2	Catalytic subunit of protein phosphatase 2 A	CGGAACAGTTCGACGAGATCATGG	141	92.3	0.996
YLS	Yellow leaf specific	CGACTGGGATGAGACTTGT	234	98.4	0.993
YLS	Yellow leaf specific	CTGTTTGTCCTTGAGAGCC	234	98.4	0.993
PP2A3	Protein phosphatase 2 A regulatory subunit	TTGAAGATTACTCGGTTTAG	116	101.3	0.999
PP2A3	Protein phosphatase 2 A regulatory subunit	ACGGGAGTGGATAAGGTA	116	101.3	0.999
EIF4α	Eukaryotic initiation factor 4 alpha	ATGCATATGGTTTTGAGAAGCC	115	92.4	0.993
EIF4α	Eukaryotic initiation factor 4 alpha	AAAGTTGCAGTCTTCCCAGTTC	115	92.4	0.993
TUA	Tubulin α	GGTGGTGGAACTGGCTCTG	217	95.0	0.989
TUA	Tubulin α	GGCAAATGTCATAGATGGCTT	217	95.0	0.989
eIF	Eukaryotic translation initiation factor	GGTCCATCCTCATACATC	102	92.9	0.983
eIF	Eukaryotic translation initiation factor	GCTATCACTTCACTCCCAC	102	92.9	0.983
vps	Vacuolar protein sorting-associated protein	GAGGTGATGGGTGATGC	134	95.5	0.984
vps	Vacuolar protein sorting-associated protein	CCTGAGGGAGCGTTTACT	134	95.5	0.984
PHD	PHD finger protein	GGCGTCAGGATTCCACAG	123	98.2	0.992
PHD	PHD finger protein	AAGCTGCAAGGTAATGTTCG	123	98.2	0.992
GTP	GTP-binding protein	GTTCAACACCAGCCAACT	92	101.3	0.998
GTP	GTP-binding protein	ATCTGATGACCACCCAAG	92	101.3	0.998

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