Fig. 5 | Scientific Reports

Fig. 5

From: Genome-wide screening and validation of exosome-derived TLN1 as a regulator of epithelial–mesenchymal transition in lung cancer

Fig. 5

Lung cancer cell exosome derived- TLN1 promotes lung cancer progression by driving EMT. (A) TLN1 expression was determined in lung cancer cells and normal lung epithelial cells by RT-qPCR. (B) The efficiency of TLN1 silencing with siRNA in A549, HCC827 and NCI-H520 cells was evaluated by RT-qPCR and Western blot. (C,D) Transwell assay was used to evaluate the migration and invasion in A549, HCC827 and NCI-H520 cells after TLN1 inhibition. (E) RT-qPCR detects the relative expression levels of E-cadherin, N-cadherin, Vimentin and ZEB1 relative to those of GAPDH in A549, HCC827 and NCI-H520 cells after TLN1 inhibition. (F) Western blot analysis of the expression levels of E-cadherin, Vimentin, total AKT, total FAK, phosphorylated AKT and phosphorylated FAK in A549, HCC827 and NCI-H520 /Ctrl and TLN1 knockdown cells. (G) Transwell assay was used to evaluate the migration and invasion in BEAS-2B cells treated with PBS, HCC827 siCtrl/EV and siTLN1/EV. (H) Western blot analysis of the expression levels of E-cadherin, Vimentin, total AKT, total FAK, phosphorylated AKT and phosphorylated FAK in BEAS-2B cells treated with PBS, HCC827 siCtrl/EV and siTLN1/EV. In bar graphs, values are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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