Fig. 2

GlcN increased FGF21 expression in a time-dependent manner. AML12 cells were treated with GlcN (10 mM) for the indicated times (0, 2, 4, 8, 12, and 24 h). FGF21 expression was analyzed in terms of (a) cell content, (b) secretion, and (c) mRNA levels. Data are presented as mean ± SEM. *P < 0.05 compared with control (n = 3).