Fig. 3 | Scientific Reports

Fig. 3

From: Glucosamine induces hepatic FGF21 expression by activating the Akt/mTOR/p70S6K axis and driving PGC-1α activity

Fig. 3

GlcN induced FGF21 expression via the Akt/mTOR/p70S6K pathway. To block Akt/mTOR/p70S6K signaling, cells were pretreated with LY294002 (10− 5 M), rapamycin (10− 7 M), or PF4708671 (10− 5 M) for 1 h, followed by treatment with 10 mM GlcN for 24 h. FGF21 expression was assessed in terms of (a) cell content, (b) secretion, (c) mRNA, and (d) protein levels. Data are presented as mean ± SEM. *P < 0.05 compared with control; #P < 0.05 compared with GlcN alone (n = 3).

Back to article page