Fig. 4
GlcN induced FGF21 expression mediated through PGC-1α. Cells were pretreated with various transcriptional regulator inhibitors (GW6471, GW9662, KC7F2, or SR18292; each at 10− 5 M) for 1 h, followed by exposure to GlcN (10 mM) for 24 h, or co-incubated with different concentrations of ZLN005 (0, 2, 4, 6, 8, 10 µM) for 24 h. (a, b) FGF21 protein levels were measured. Hepatocytes were incubated with GlcN (10 mM) for 24 h. (c) PGC-1α expression was assessed by western blot. Data are presented as mean ± SEM. *P < 0.05 compared with control; #P < 0.05 compared with GlcN alone (n = 3).
