Fig. 5
F1/F3 treatment of HeLa cells for 1 h does not affect the expression of endogenous and exogenous apoptosis-related proteins. (A) Flow cytometric analysis and quantification of cell death by annexin V and propidium iodide (PI) staining. Early apoptosis is represented by Q3 (FITC + PI-) and late apoptosis, necrosis, or pyroptosis is represented by Q2 (FITC + PI+). (B)-(H): HeLa cells (1.0 × 106) were treated with F1/F3 (10 µg/ml), P3, or left untreated for 1 h, and protein expression levels of apoptotic-related proteins were measured by Western blot. (B) Caspase-8. (C) Cleaved caspase-8. (D) Bax. (E) Cytochrome c. (F) Apaf-1. (G) Caspase-7. (H) Cleaved caspase-7. These results come from a representative experiment in which two independent experiments were conducted. All data are presented as the mean ± SEM. *P-value < 0.05, **P-value < 0.01, ***P-value < 0.001. Statistical significance was assessed using one-way ANOVA.
