Fig. 4

Identification of “marker” features specific to the microbiotas of the two mouse colonies before AOM injection (the “Before” time point) and after three rounds of DSS treatment (the “cDSS” time point). (A,B) LefSE analysis of the marker features at the “Before” and “cDSS” time points (n = 8−13), LDA score ≥ 2 and adjusted p-value < 0.05. Features are named by their taxonomy with unique feature identifiers. (C,D) Heatmap showing the abundance of marker features at the “Before” and “cDSS” time points (n = 8−13). Samples are clustered by Bray-Curtis distances. (E,F) Correlation heatmap representing the Spearman correlation (ρ > |0.5|) between the abundance of the identified markers at the “Before” and “cDSS” time points with the number of tumors observed at the “CRC” time point. Correlations that are not significant are crossed out. *, **, *** Benjaminni-Hochberg adjusted ρ-value < 0.05, 0.01 and 0.001.