Fig. 2 | Scientific Reports

Fig. 2

From: Htra4 promotes vascular endothelial cell injury and is associated with the early-onset of preeclampsia

Fig. 2

Htra4 facilitates the initiation of oxidative stress in HUVECs. (A, B) The impact of recombinant Htra4 protein on MDA and GSH production in HUVECs (n = 6); (C) The impact of recombinant Htra4 protein on ROS production in HUVECs (n = 6), and the scanning statistics regarding the fluorescence intensity of ROS; (D) Cellular immunofluorescence analysis of NRF2 nuclear translocation in recombinant Htra4 protein-induced HUVECs (n = 6), and the ratio of the fluorescence intensity of the nucleus to that of the cytoplasm; (E,F) Western blot analysis of NRF2/HO-1/NQO-1 expression in recombinant Htra4 protein induced-HUVECs (n = 3). Means ± SEM, ** p < 0.01, and *** p < 0.001.

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