Fig. 1 | Scientific Reports

Fig. 1

From: Unstable laboratory Wolbachia strain w-Anga is negatively correlated with Plasmodium falciparum in wild malaria vectors

Fig. 1

Mosquito samples collected have been to 16SrRNA Nested PCR to obtain amplicons positive for Wolbachia strain w-Anga. These amplicons underwent 16SrRNA gene sequencing by Sanger technology using GENEWIZ/AZENTA’s internal formulation of BigDye V3 chemistry on an ABI3730xl sequencer. The sequences obtained were subjected to phylogenetic analysis, which involved aligning them with data available in the GenBank database using BLAST. Following the BLAST alignment, we established a phylogenomic relationship between our samples (five strains, including four derived from our 16SrRNA amplicons and a positive control of wAlbB strain) and 33 Wolbachia strains from NCBI by constructing a phylogenetic tree using the “Fasttree” program in the “Jupyter Notebook” application, version 4.2.4. This phylogenetic tree was visualized and annotated using “ITOL v6.9.1.” The assembly names on the phylogenetic tree were color-coded based on the identity of the supergroups (A-F) and other 16SrRNA sequences.

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