Fig. 4

Volcano plot and random forest plot for pairwise comparison of differential metabolites in the four groups of mice. Volcano plot of the comparison of differential metabolites in ND VS HFD in positive (A) and negative (B) ion mode. Volcano plot of the comparison of differential metabolites of HFD VS HFD-ACT001 in the positive ion mode (D) and negative ion mode (E). Volcano plot of the HFD VS HFD-PPC differential metabolites in the positive ion mode (G) and negative ion mode (H). Random forest plots of differential metabolites in the positive and negative ion modes of ND VS HFD (C), HFD VS HFD-ACT001 (F), and HFD VS HFD-PPC (I).