Fig. 3
From: Development of an iridium complex fluorescent probe for FGF21 protein labeling and tracking
Binding specificity of IrCN and FGF21 protein with His tag. (A) Schematic diagram of protein molecules FGF21 with His tag and bovine serum albumin (BSA). The molecular weight of FGF21 and BSA is 22 kD and 66 kD, respectively. The blue line represents the position of his in the polypeptide chain. (B) Each sample tube with addition of IrCN probe, FGF21 and BSA protein in turn. The mixture after reaction for 15 min were subjected for gel electrophoresis and fluorescence detection, respectively.
