Fig. 4
(a, b) The knockdown levels of MLH1 in Hep 3B cell line were detected by qPCR and western blot. β-actin was used as internal parameter. (c) Cell proliferation capacity was determined by CCK-8 analysis after knockdown of MLH1 in Hep 3B cells. (d) Effect of inhibition of MLH1 expression on colony formation of Hep 3B cells. (e) Effect of inhibition of MLH1 gene expression on apoptosis of Hep 3B cells (f) Wound healing assays revealed that knockdown MLH1 notably inhibited migration ability of Hep 3B cells (100 ×). (g) Transwell assays revealed that knockdown MLH1 notably inhibited migration and invasion of Hep 3B cells (100 ×). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
