Fig. 3
Marein restored SIRT1/Nrf2 signaling in H2O2-induced HepG2 cells. HepG2 cells were exposed 500μM H2O2 condition for 24h, and then administrated by 5 μM Marein for another 24h. (a, b) mRNA expression levels of SIRT1 (a) and Nrf2 (b) were assessed using RT-qPCR. (c) Western blot analysis of SIRT1 and Nrf2 protein levels. (d) Immunofluorescence staining of Nrf2 nuclear localization (scale bar = 25 μm). Data are represented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
