Fig. 8
In vitro experiments to explore the role of PRC1 in WIT-49 cells. (A) The relative mRNA expression levels of PRC1 in HEK293T, WIT-49, and SK-NEP-1 cell lines detected by RT-qPCR (p < 0.05). (B) Verification of PRC1 knockdown efficiency in WIT-49 cells transfected with shPRC1-1 and shPRC1-2 plasmids compared to the control group by RT-qPCR (p < 0.05). (C) Fluorescence photos under a microscope to obtain the transfection efficiency of WIT-49 cells. (D, E, F) Assessment of cell migration ability: measurement of the migration area of each group at 24 and 48 h after scratching (p < 0.05). (G, H) Transwell assay indicating cell invasion ability (p < 0.05). (I) Cell viability measured by CCK-8 assay, revealing lower OD values at 450 nm in shPRC1-1 and shPRC1-2 groups on days 3 and 4 compared to the control group (p < 0.05). (J, K, L) The expression levels of EMT-related core proteins detected by western blotting (p < 0.05). (M) Measurement of glucose levels in WIT-49 cells. (N) Measurement of lactic acid levels in WIT-49 cells. (O) BSA protein standard curve: Y = 1.107*X + 0.1883, R2 = 0.9914. (P) Comparison of protein sample concentrations in Control, shPRC1-1, and shPRC1-2 groups.
