Fig. 2

Downregulation of AJUBA attenuated the proliferation of HCC cells. (A) Protein expression levels of AJUBA in normal lung epithelial cells (HBE) and NSCLC cell lines (H1299, A549, H460 and H1650). One-way ANOVA was used to analyze the differences in grayscale among different groups. (B) The mRNA levels of AJUBA in AJUBA-knockdown H1299 and A549 cells. Two-tailed Student’s t-test was used to analyze the differences between the two groups. NC, negative control. ***P < 0.001. (C) The protein levels of AJUBA in AJUBA-knockdown H1299 and A549 cells. One-way ANOVA was used to analyze the differences in grayscale among different groups. (D) Cell viability was detected in AJUBA-knockdown H1299 and A549 cells. Effect of AJUBA knockdown on cell growth of H1299 and A549 cells was analyzed by colony formation assay. Two-tailed Student’s t-test was used to analyze the differences between the two groups,. ^**P < 0 01, ^***P < 0.001. (E) Effect of AJUBA knockdown on cell growth of H1299 and A549 cells was analyzed by colony formation assay. Two-tailed Student’s t-test was used to analyze the differences between the two groups. ^**P < 0.01.