Fig. 4
Elevated AJUBA expression was positively associated with β-catenin expression in NSCLC and regulated the expression of EMT-related proteins. (A) Correlation of the protein level of AJUBA and β-catenin in NSCLC tumor tissues by IHC (200X). (B) Western blotting analysis for AJUBA, Cyclin D1, MMP-9, β-catenin, N-cadherin and Vimentin in H1299 and A549 cells after transfection with or without siAJUBA, GAPDH served as an internal control. One-way ANOVA was used to analyze the differences in grayscale among different groups. (C) Western blotting analysis was employed to detect the protein level of AJUBA, p-ERK, β-catenin, N-cadherin and Vimentin in H1299 and A549 cells with AJUBA overexpression and/or PD98059 treatment. One-way ANOVA was used to analyze the differences in grayscale among different groups.
