Fig. 1
From: Optimization of SgRNA expression with RNA pol III regulatory elements in Anopheles stephensi
Crossing scheme for inheritance assessments. cdsgRNA females were crossed to zpg3’Cas9 males. The transheterozygous F1 were then crossed either to WT or to cd−/− of the opposite sex. The F2 generation was screened for the presence of the fluorescent markers as well as eye phenotype.
