Fig. 1
From: Establishment and genetic characterization of zebrafish RW line
Schematic illustration of the maintenance protocol for the RW line. (A) During each breeding cycle, two pairs of adult zebrafish were housed together in a mating tank to produce embryos. The embryos were screened for developmental abnormalities or mortality up to 5 dpf. Only healthy clutches in which the majority of embryos exhibited normal development by 5 dpf were selected for rearing to adulthood. (B) After reaching maturity, healthy adult fish were used for subsequent breeding. This selection process effectively eliminated harmful genetic variants associated with developmental defects, fragility, and reduced fertility. Breeding has been conducted approximately once a month to sustain the RW line.
