Fig. 4

Real-time detection of the F290 V and T929I mutations in Spodoptera frugiperda by an allele-specific real-time PCR fluorescent probe in West Africa. Bivariate plot showing the discrimination of the acetylcholinesterase (F290 V) allele in Benin (a), Togo (b) and Niger and Mali (c). Bivariate plot showing the discrimination of the voltage-gated sodium channel (VGSC) (T929I) allele in Benin, Togo, Niger and Mali (d). Each dot represents a single larva. The blue circle represents susceptible SS homozygotes (F290; allele 2), the red lozenge mutant RR homozygotes (V290; allele 1) and the green square RS heterozygotes (F290/V290).