Fig. 4

Effects of HFD and CRS on the phenotypic change of astrocytes. (A–D) Representative confocal images labeled with GFAP (red), C3 (green) and S100A10 (green) in the DG region of hippocampus. Scale bar, 50 μm (× 20 magnifications). (E–H) Representative for western blotting photograms for GFAP, C3, and S100A10 in the mouse hippocampus of the control, HFD, CRS, and HFD + CRS groups. The expression values of GFAP (F), C3 (G), and S100A10 (H) were normalized with β-actin value. Data were presented as mean ± SEM (n = 3). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. CRS group; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs. HFD group; n.s. not significant.