Fig. 6

Effects of PA and Cort stimulation on the phenotypic change of C8-D1A astrocytes. (A) Schematic diagram showing treatment of C8-D1A astrocytes with PA and Cort. (B,C) Effect of Cort and PA on the viability of C8-D1A astrocytes. (D,E) C3⁺/GFAP⁺ expression in C8-D1A astrocytes detected by immunofluorescence, scale bar, 50 μm (× 40 magnifications). (F,G) S100A10⁺/GFAP⁺ expression in C8-D1A astrocytes detected by immunofluorescence, scale bar, 50 μm (× 40 magnifications). (H–K) Representative for western blotting photograms for GFAP, C3, and S100A10 in C8-D1A astrocytes of the control, PA, Cort, and PA + Cort groups. The expression values of GFAP (I), C3 (J), and S100A10 (K) were normalized with β-actin value. Data were presented as mean ± SEM (n = 3). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. Cort group; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs. PA group; n.s. not significant.