Fig. 7

Effect of HFD combined with CRS on the Wnt/β-catenin signaling pathway. (A–D) Representative for western blotting photograms for Wnt3a, β-catenin, and p-GSK3β /GSK3β in the hippocampus of the control, HFD, CRS, and HFD + CRS groups. Wnt3a (B) and β-catenin (D) expression values were normalized with the β-actin value, respectively. The expression values of p-GSK3β (C) were normalized with GSK3β value. (E,F) Wnt3a (green) expression in the hippocampus was detected by immunofluorescence, Scale bar, 50 μm (× 20 magnifications). Data were presented as mean ± SEM (n = 3). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. CRS group; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs. HFD group.