Table 2 Live/dead cell assay in flow cytometry.
From: Influence of biopolymer based gold nanoparticles and photobiomodulation in in vitro wound healing
Quadrant Detail | Staphylococcus aureus (in %) | Pseudomonas aeruginosa (in %) | |
|---|---|---|---|
Unstained cells | Q1—Unstained | 100 | 99.8 ± 0.2 |
Q2—Live (TO) | 0 | 0.05 ± 0.05 | |
Q3—Injured (TO/PI) | 0 | 0 | |
Q4—Dead (PI) | 0 | 0 | |
TO/PI-Stained Positive Control Treated | Q1—Unstained | 19.9 ± 0.8 | 44.6 ± 2.1 |
Q2—Live (TO) | 68.8 ± 5.5 | 38.35 ± 1.25 | |
Q3—Injured (TO/PI) | 11.25 ± 4.75 | 4.4 ± 0.9 | |
Q4—Dead (PI) | 0.1 | 12.7 ± 0.1 | |
TO/PI-Stained GT Treated | Q1—Unstained | 7.65 ± 0.55 | 71.55 ± 1.05 |
Q2—Live (TO) | 73.85 ± 0.95 | 17.45 ± 0.25 | |
Q3—Injured (TO/PI) | 18.4 ± 0.4 | 4.4 ± 1.9 | |
Q4—Dead (PI) | 0.1 | 6.6 ± 1.1 | |
–TO/PI-Stained GT-GNPs Treated | Q1—Unstained | 19.75 ± 0.15 | 69.2 ± 1 |
Q2—Live (TO) | 62.5 ± 1.2 | 23.15 ± 0.35 | |
Q3—Injured (TO/PI) | 17.65 ± 1.35 | 3.45 ± 1.25 | |
Q4—Dead (PI) | 0.1 | 4.2 ± 0.7 |
