Table 4 Levels of independent variables used in Box-Behnken design (BBD) for optimizing laccase production (U L− 1) from Virgibacillus salarius.
Independent variable | Unit | Symbol | Coded variable level | ||
---|---|---|---|---|---|
Low (− 1) | Medium (0) | High (+ 1) | |||
Sodium chloride | M | X1 | 1 | 2 | 3 |
pH | X2 | 6 | 7 | 8 | |
Peptone | % | X7 | 0.5 | 1 | 1.5 |
Copper sulfate | mM | X9 | 3 | 5 | 7 |