Table 4 Levels of independent variables used in Box-Behnken design (BBD) for optimizing laccase production (U L− 1) from Virgibacillus salarius.

From: Green valorization of garlic peel waste using halophilic laccase for efficient biomass delignification and biorefinery applications

Independent variable

Unit

Symbol

Coded variable level

Low (− 1)

Medium (0)

High (+ 1)

Sodium chloride

M

X1

1

2

3

pH

 

X2

6

7

8

Peptone

%

X7

0.5

1

1.5

Copper sulfate

mM

X9

3

5

7