Table 6 Analysis of variance (ANOVA) for a quadratic model of Box-Behnken design (BBD) for optimizing laccase production (U L−1 from V. salarius.
Source | Mean squares | Df | F-Value | p-Value |
|---|---|---|---|---|
Model | 2.2 × 105 | 14 | 1747.4 | < 0.0001 |
NaCl (M) | 12404.1 | 1 | 1343.0 | < 0.0001 |
pH | 62075.6 | 1 | 6721.0 | < 0.0001 |
Peptone (%) | 15589.5 | 1 | 1687.9 | < 0.0001 |
CuSO4 (mM) | 79028.7 | 1 | 8556.6 | < 0.0001 |
pH × NaCl | 1866.2 | 1 | 202.0 | < 0.0001 |
pH × Peptone | 508.9 | 1 | 55.1 | 508.9 |
pH × CuSO4 | 4080.7 | 1 | 441.8 | < 0.0001 |
NaCl × Peptone | 2246.8 | 1 | 243.3 | < 0.0001 |
NaCl × CuSO4 | 6290.9 | 1 | 681.1 | < 0.0001 |
Peptone × CuSO4 | 11193.6 | 1 | 1211.9 | < 0.0001 |
(pH)2 | 1284.2 | 1 | 139.0 | < 0.0001 |
(NaCl)2 | 19244.7 | 1 | 2083.6 | < 0.0001 |
(Peptone)2 | 0.04 | 1 | 0.004 | 0.9472 |
(CuSO4)2 | 5546.1 | 1 | 600.5 | < 0.0001 |
Residual | 129.3 | 14 | ||
Lack of Fit | 129.3 | 10 | 0.1742 | |
Pure Error | 0.0 | 4 | ||
Cor Total | 2.3 × 105 | 28 |
