Fig. 4
Cytometric bead array quantification of cell models cytokine secretion in response to stimuli. Primary human microglia, iPSC-derived microglia, human pericytes, and mouse microglia were treated with inflammatory mediators (LPS, IL1β, IFNγ, TNFα) or vehicle (0.1% BSA in PBS) for 24 h and conditioned media collected for cytometric bead array or Griess assay analysis. Cytokine secretion data are represented as heat maps for each cell model, scale bar log2 concentration in pg/ml/10,000 cells (A–E). Quantified secretion of cytokines and chemokines by each cell model (E1 - E10) n = 3–5, pg/ml/10,000 cells. Quantified nitric oxide (NO) secretion (µM) (F). n = 3 independent cases, data presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparison test comparing vehicle with each treatment within each cell type * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.
