Table 4 Analysis of variance (ANOVA) for the Box–Behnken model optimizing Phlorotannin extraction from D. justii.

From: Optimization of ultrasound extraction of phlorotannin rich extract from Dictyopteris justii with tyrosinase Inhibition

Source

DF

SS

MS

F Value

p Value

Model

14

12.3780

0.8841

36.71

0.000

Linear

4

11.4202

2.8551

118.53

0.000

Solvent

1

10.9271

10.9271

453.66

0.000

S: L Ratio

1

0.1736

0.1736

7.21

0.023

Temperature

1

0.2848

0.2848

11.83

0.006

Time

1

0.0132

0.0132

0.55

0.476

Square

4

0.1117

0.0279

1.16

0.385

Solvent * Solvent

1

0.0022

0.0022

0.09

0.771

S: L Ratio * S: L Ratio

1

0.0000

0.0000

0.00

0.974

Temperature * Temperature

1

0.0852

0.0852

3.54

0.089

Time * Time

1

0.0000

0.0000

0.00

0.976

2-Way Interaction

6

0.6808

0.1135

4.71

0.016

Solvent * S: L Ratio

1

0.0809

0.0809

3.36

0.097

Solvent * Temperature

1

0.2921

0.2921

12.13

0.006

Solvent * Time

1

0.0071

0.0071

0.30

0.598

S: L Ratio * Temperature

1

0.0028

0.0028

0.12

0.740

S: L Ratio * Time

1

0.2733

0.2733

11.35

0.007

Temperature * Time

1

0.0474

0.0474

1.97

0.191

Error

10

0.2409

0.0241

  

Lack of fit

8

0.2118

0.0265

1.82

0.402

Pure error

2

0.0291

0.0145

  

Total

24

12.6189

   
  1. DF: Degree freedom; SS: Sum of Squares; MS: Mean Square.